What role does topoisomerase play during DNA replication?

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Unwinding the DNA double helix

Synthesizing RNA primers

Relieving torsional strain ahead of the replication fork

Sealing nicks in the DNA backbone

Answer: Relieving torsional strain ahead of the replication fork

Explanation:

Topoisomerase enzymes prevent supercoiling and relieve the torsional strain that builds up ahead of the replication fork during DNA replication.